**Please note time/location change for this seminar.
Manitoba Neuroscience Network
Seminar Series
Friday, February 22nd, 2013 | 2:00 pm
Dr. Derek van der Kooy
Professor
Department of Molecular Genetics
University of Toronto
Topic: Where Brains Come From
Location: Theatre B
Derek van der Kooy served as Professor in the Department of Anatomy and Cell Biology at the University of Toronto from 1991 until 2002, when he became a Professor in the Department of Molecular Genetics. Derek received a M.Sc. in Psychology at the University of British Columbia, and a Ph.D in Anatomy, first at Erasmus University in the Netherlands, and finishing in the Department of Anatomy at the University of Toronto. Dr. van der Kooy gained postdoctoral research experience at Cambridge University in England and at the Salk Institute in California.
The van der Kooy lab works on various stem cell biology and developmental biology research projects; specifically, stem cells in organisms from Drosophila to humans. We produced the first report of stem cells in the adult mammalian eye, published in 2000 in Science. We also have isolated a rare stem cell from the adult mouse and human pancreas that can show extensive proliferation under defined conditions in vitro. Of interest to the lab is the lineage of neural stem cells from pluripotent embryonic stem cells, with relevance to the origin of the earliest neural stem cell in the developing embryo.
ABSTRACT: One of the few neurobiological facts not anticipated by Cajal is the existence of neural stem cells in the embryonic and adult mammalian brain that can produce new neurons in the adult brain (new neurons first shown by Altman). The earliest mammalian neural stem cells differentiate from pluripotent embryonic stem cells. These primitive neural stem cells emerge in response to LIF and have a wider non-neural phenotypic potential than later neural stem cells. By embryonic day 8.5 in mouse, true FGF2 dependent neural stem cells emerge and by embryonic day 14 they have given rise to copies of themselves and to EGF dependent neural stem cells. The separate FGF2 and EGF dependent neural stem cells increase greatly in numbers later in neurogenesis, but by E14 appear identical to adult neural stem cells. The adult mammalian neural stem cells are mostly quiescent, dividing asymmetrically only once every few weeks.
Mouse embryonic stem (ES) cells cultured in low cell density, completely defined media adopt a neural identity. Using a clonal colony-forming assay, we identify the novel primitive neural stem cell stage as a component of neural lineage specification (this cell is similar to the one we isolate from the early embryo), which is negatively regulated by TGFb-related signaling. These results are consistent with a default mechanism for neural fate specification. Primitive neural stem cells are formed directly from single ES cells in a LIF-dependent manner, express multiple neural precursor markers and give rise to neurons and glia. Moreover, in vivo mouse chimera experiments reveal that these primitive ES-derived neural stem cells have a broad range of neural and non-neural lineage potential. These results support a model whereby definitive neural stem cell formation is preceded by a primitive neural stem cell stage during neural lineage commitment. Most recently, we have found that the LIF-dependent, primitive neural stem cells persist in adult organisms and can repopulate a depleted definitive neural stem cell pool in the adult.
For more information, contact the MNN Office at
(T) 235.3939 or email: mnn@sbrc.ca
Presented in co-operation with University of Manitoba
Clinical Neuroscience Rounds
Kelly Jorundson
Winnipeg Chapter Society for Neuroscience
R4046 - 351 Tache Avenue
Winnipeg, MB R2H 2A6
Tel: 204.235.3939
Fax: 204.237.4092
Email: kjorund@sbrc.ca OR kjorund@yahoo.ca
From: Krystyna Kruczek [mailto:KKruczek@exchange.hsc.mb.ca]
Sent: February 12, 2013 10:48 AM
To: Kelly Jorundson
Subject: FW: Neuroscience Seminar Series - Feb. 22nd
Hi Kelly,
I am sending out the notice for February 22nd Neuroscience Rounds as Marie is away. Can you email me the information I need on the Neuroscience Seminar Series for that day?
Thanks.
Krys Kruczek
Administrative Assistant to,
Neil Berrington, MMed, FRCS, FCS (SA)
Assistant Professor
Head - Section of Neurosurgery
Phone: 204-787-7261
Fax: 204-787-3851
Email: KKruczek@exchange.hsc.mb.ca
P Please consider the environment before printing this email
From: Marie Field
Sent: Wednesday, February 06, 2013 9:39 AM
To: Krystyna Kruczek
Subject: Neuroscience Seminar Series
Hi Krys,
I receive info re the Neuroscience Seminar Series from:
Kelly Jorundson
Winnipeg Chapter Society for Neuroscience
R4046 - 351 Tache Avenue
Winnipeg, MB R2H 2A6
Tel: 204.235.3939
Fax: 204.237.4092
Email: kjorund@sbrc.ca OR kjorund@yahoo.ca
I appreciate you contacting her for the February 22nd info and for sending out the Neuroscience Rounds notice while I am away.
_______________________________
Marie Field
Education Coordinator
Dermatology, Infectious Diseases, Neurology
and Physician Assistant Program
Department of Internal Medicine
University of Manitoba
GF328, Health Sciences Centre
Tel: (204) 787-8624 l Fax: (204) 787-4528